Improved detection of differentially expressed genes in microarray experiments through multiple scanning and image integration
نویسندگان
چکیده
منابع مشابه
Selecting differentially expressed genes from microarray experiments.
High throughput technologies, such as gene expression arrays and protein mass spectrometry, allow one to simultaneously evaluate thousands of potential biomarkers that could distinguish different tissue types. Of particular interest here is distinguishing between cancerous and normal organ tissues. We consider statistical methods to rank genes (or proteins) in regards to differential expression...
متن کاملDetecting differentially expressed genes in longitudinal microarray experiments
In this work we propose a statistical approach for the detection of differentially expressed genes. Specifically, we envisage a longitudinal study design where multiple biological replicates are available and each individual is observed at more than one time point. We present a semi-parametric random effects model based on smoothing splines and introduce a visual-distance metric for detecting t...
متن کاملSample size for detecting differentially expressed genes in microarray experiments
Background: Microarray experiments are often performed with a small number of biological replicates, resulting in low statistical power for detecting differentially expressed genes and concomitant high false positive rates. While increasing sample size can increase statistical power and decrease error rates, with too many samples, valuable resources are not used efficiently. The issue of how ma...
متن کاملIdentifying Differentially Expressed Genes in cDNA Microarray Experiments
A major goal of microarray experiments is to determine which genes are differentially expressed between samples. Differential expression has been assessed by taking ratios of expression levels of different samples at a spot on the array and flagging spots (genes) where the magnitude of the fold difference exceeds some threshold. More recent work has attempted to incorporate the fact that the va...
متن کاملIdentifying differentially expressed genes from microarray experiments via statistic synthesis
MOTIVATION A common objective of microarray experiments is the detection of differential gene expression between samples obtained under different conditions. The task of identifying differentially expressed genes consists of two aspects: ranking and selection. Numerous statistics have been proposed to rank genes in order of evidence for differential expression. However, no one statistic is univ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2003
ISSN: 1362-4962
DOI: 10.1093/nar/gng149